Which of the following would probably not be affected when a protein is denatured?

  1. the folded shape of the protein

  2. tertiary structure

  3. secondary structure

  4. quaternary structure

  5. primary structure



Denaturation affects the tertiary structure of a protein as well as having the potential to alter the R-groups involved in secondary folding. The primary structure of amino acid sequencing stands to be the least likely to be affected by changes in pH or temperature.